Bradi1g72430 was hypothesized to play a role in cell wall biogenesis, so it was expected to be more highly expressed with increase tissue growth. We hypothesized that if Brachypodium distachyon plants were treated with indole-3-acetic acid (IAA), which stimulates root growth, we would see increased gene expression due to the increased root growth and subsequent formation of cell walls.
We set up two 30 mL agar plates, one with 1.5 µL of ethanol (control) and one with 1.5 µL of 0.0001M IAA for a final concentration of 5 nM IAA (treated). Once set, four B. distachyon seeds were planted on each plate, and the plates were placed in the incubator for seven days, covered by a blue-minus filter, before the entire root of each plant was removed for RNA extraction. The four control plants were labeled as samples 1-4 while the four treated plants were labeled as samples 5-8. The extracted RNA was quantified with NanoDrop (Table 3) and visualized via agarose gel electrophoresis (Figure 7).
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