The samples treated with RNase only displayed the band of DNA while the samples untreated with RNase displayed a band of DNA and a band of much smaller RNA further from the wells. . From this we can conclude that the RNA contained within the extraction was eliminated in the RNase-treated samples.
For the sample treated with RNase, the ratio of the absorbance at 260 nm divided by the reading at 280 nm was lower than the expected 1.8 at 1.26, indicating that the DNA extraction was not very pure and may have contained contaminants. The sample not treated with RNase had a ratio of 2.08, which indicates the DNA was contaminated with RNA, which has a larger ratio than pure DNA. The concentration after eliminating RNA with RNase was 0.2590 µg/µL, meaning there was 12.95 µg of DNA present in the original 50 µL extraction solution.
Strangely, the concentration of nucleic acids was 259.0 ng/µL for the DNA treated with RNase, which is higher than the 200.7 ng/µL concentration of DNA untreated with RNase. The concentration is expected to be lower once the RNA is eliminated. This can be due to a unequal dividing of the genomic DNA before treating one sample with RNase.
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