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Draft: Lab 2 Results I

Submitted by aspark on Tue, 03/26/2019 - 00:48

The Phytozome locus page for the unknown gene described the product as a leucine-rich repeat protein kinase in the LRR-V subfamily. On the UniProt page, several preliminary predictions were shown: Transmembrane receptor protein serine/threonine kinase activity and ATP binding were listed as functions, and protein phosphorylation was listed as the biological process. The plasma membrane was listed as the cellular location.

    The Nucleotide BLAST searching for DNA-level similarities resulted in one match with more than five exons within the coding region; the mRNA sequence for Brachypodium distachyon protein SRF7 (XM_003558607.4) had 12 exons and was the 12th sequence from the top in the list (Figure 3). There was also a short sequence roughly from bases 4060-4340, within exon 11 of Bradi1g72430, that was present in every match. All observed matches were from monocot plant species, including Oryza sativa Japonica, Brachypodium distachyon, Zea mays, and Triticum aestivum.

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