The purpose of this lab is to determine how single nucleotide polymorphisms in the Canis Lupus Familiaris genome can be linked to specific traits. Dogs are perfect candidates to study how changes in ones genotype relates to phenotypic variation. This was accomplished by isolating canine DNA, designing primers to amplify desired single nucleotide polymorphisms, running PCR and digesting the DNA sequences with a restriction enzyme.The products of the digestion were then run on a gel to determine the alleles of each trait. Gel electrophoresis yielded no discernable result or conclusions due to possible errors with load dye or restriction enzymes.
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