DNA-DNA hybridization is a method used to estimate the amount of genetic changes that took place in the genome as a whole since the time at which two groups diverged from their most closest ancestor. The 4 nucleotides has 4 unique base A, C, G, and T, these base pairs would allow scientists to analyze how similar each species are to one another. Fred Sheldon was one of the pioneers to perform this experiment and he confirmed that Great Blue Heron is similar to Great Egret than American Bittern.
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