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Confirming Mutation Discussion

Submitted by ewinter on Thu, 04/18/2019 - 19:49

The bright bands on the gel indicate that DNA was successfully extracted. This was expected, despite some worry about self complementation due to the “any th” value of 8.16 in the primer3 output. We do not know why lane number 2 on the TE gel did not show a band. We recall loading sample into this lane. It is possible that something in the DNA extraction protocol failed, so no DNA of large band size was extracted. We preferentially chose mutants from the TE gel to send for sequencing because TE stabilizes DNA. All three of the trustworthy sequence results confirmed that the mutant plants were homozygous for the NaN1793 mutation.

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