cdc20 and Naegleria

You could use antibodies specific to cdc20 protein to see if it is expressed in the Naegleria. The primary antibodies would bind to cdc20 (if it is present) and the secondary antibodies would bind to the constant region of the primary antibodies. Using polyclonal antibodies as the primary antibody would be a good idea because they can detect low expressing proteins. If we detect cdc20 protein in our experimental group, then the gene silencing and RNAi didn't work. If we do not detect cdc20, then it was silenced, which is what we expect. In our negative control, since cdc20 is not silenced, we expect detection of it. Antibodies can therefore help us make sure that any changes in phenotype between the negative control and the experimental group are because we silenced cdc20.