ELISA will also for HK2 and PKM2, as these should be downregulated if the Ginsenoside 20(S)-Rg3 treatment worked. Since ovarian cancer metastasizes so quickly and mutates in unpredictable manners, we would biopsy each site of metastasis for the primary tumors, and each new metastasis as well. The microenvironment of each individual tumor has the possibility to be different, and the biopsies will aid us in understanding if CAFs, immune cells, or other social microenvironment based cells are becoming amplified. The biopsy results from distant metastatic sites will also provide insight into what kind of mutations the cancer is undergoing.
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