This past week in lab, we performed computer tasks to determine the bioinformatics of the fish samples we previously isolated DNA and performed PCR on. We began by assessing the quality of the DNA samples—our tuna was of excellent quality and our haddock was of poor quality. We assessed the mean quality scores and standard deviation for both directional sequences. From viewing each trace file, we searched the BOLD database for a match in the forward and reverse sequences. A match was found with our tuna sample, displaying 100% similarity to Thunnus alalunga. Our poorer quality fish sample, haddock, displayed a 96.1% similarity match with Melanoggrammus aeglefinus. Finally, we examined both sequencing traces for failed quality data.
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