This experiment showed that biofilms from two different bacterial sources could be grown in the lab and visualized using microscopy. P. aeruginosa was shown to be an excellent biofilm former, producing a thick, gooey, green slime. The EPS and rod shaped bacteria were clearly visible under the microscope. The environmental sample taken from the soil also produced a biofilm. It was yellow in color, smelled like rotting organic material, and was not as thick and gooey as the biofilm produced by P. aeruginosa. Under the microscope EPS and bacteria were visible, showing that there were some bacteria in the soil and root sample that had the ability to produce biofilms. Keeping the biofilms wet using the flow through gram stain was essential to maintaining the structure of the biofilm and allowed for visualization under the microscope. This experiment showed that two different sources of bacteria both produced biofilms, which were visualized under a microscope using a technique that kept the biofilms wet.
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