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To make sure that the spider strictly picks a side based on substrate color is it important to keep all other factors consistent. If in the experiment the spider forms a web it will be taken out. We do not want the spider to keep choosing the same side just because there is a web there for it. Both sides of the environment should also be exactly the same except for background color.

Both sides of the environment should also be exactly the same except for background color. The temperature needs to be kept consistent inside of the tank. To do this, the spiders need to be kept away from windows and housed somewhere there is no breeze. It has been shown that spiders are less active in colder temperatures, so keeping them at room temperature (70°F) is important. Light entering from outside of the tank should also be the same on both sides. To keep this consistent do not place in sunlight, or somewhere that there are shadows. Overhead lights should be used because they will keep the light even on both sides. The lights should remain on 24 hours a day, so the spiders are always able to see the color of the background they are on.