In the crossing of the mutant strands, there were fewer colonies observered than was expected on the control plate (one-hundred and fifty). This might have been due to a dilution error. For the MV plates, there were approximately ninety colonies observed. The reason for this proportion colonies may be due to not plating the same number of cells on the control plate as there should have been. Theoretically, there should have had a 20% survival rate if the optimal exposure was achieved. For the MV plate, the survival rate was 3.3%. This is significantly lower than expected and might be due to errors as mentioned above. There were no mutant phenotypically red colonies observed. This was expected, as you need about 10,000 surviving yeast cells for each mating type (3) to observe surviving mutant colonies that express a red color. The survival rate for our control plate was 6%. This was also much lower than the 20% expected survival rate, for reasons similar to the first plate.
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