Abstract

   An experiment to determine the effects of gene complementation when a mutation occurs was performed on Saccharomyces cerevisiae, or as it is known better by its common-use name, Yeast. The mutation was induced by UV radiation, and complementation was observed by the crossing of the resulting mutants. It was found that when there is no adenine present in a growth medium, diploid yeast cells crossed from mutant haploid cells do not grow unless there is complementation. The resulting experiment shows this through plating two haploid strands of yeast, MATa, and MATα to form diploid cells.