Different types of white blood cells on eight other blood smears were analyzed and identified. We looked at these under the microscope starting with 4X magnification and moving to 10X & 40X. Our goal here was to identify as many different white blood cells as possible in each smear. We also needed to correctly create and read a hematocrit/PCV test. To make one to be observed first put hematocrit capillaries from the blood sample you want into an eppendorf tube. W tried to fill it at least half way. We did Blood C and B while our partners did Blood A. When it was filled, we put an index finger over the top of the tube and plugged the bottom with putty to seal it. We then put both tubes into the centrifuge for five minutes at 14,000 rpm. After five minutes was up, we measured the amount of red blood cells, buffy coat, and total amount of material inside of the tube in centimeters. By measuring this we calculated the hematocrit/ PVC count for the blood type. We calculated it by taking the amount of red blood cells in centimeters and dividing it by the total amount of material in the tube. This allowed us to diagnose the animals who provided the blood with healthy or unhealthy.
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