Alicia DiCicco

To begin our experiment, we had to isolate the chloroplasts. First, we took the spinach leaves and ground them in a mixture of 20 mL of grinding buffer and a pinch of sand. Once thoroughly ground we used cheesecloth to sift the liquid from the mixture into a beaker. This liquid then went into tube A. Tube A was then placed in the centrifuge for 3 minutes at 200 X G. Next the supernatant from this was put into tube B which was then placed into the centrifuge for 10 minutes at 1000 X G. This formed a hard pellet that containing thylakoids which is what we used for the experiment. We then resuspended the pellet in 5.5 mL of grinding buffer. Once suspended the solution was then placed into another beaker, so we could use it in the next part of the experiment.