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PP Yeast Mutagenesis

Submitted by jnduggan on Fri, 10/19/2018 - 14:15

The “Yeast Mutation and Analysis” lab protocol served as a guideline for the first day of our Yeast Mutagenesis experiment. On day one we performed a serial dilution of ~107to ~104yeast cells using a pipette, sterile test tubes, and vortexer. From the105dilution, we pipetted 100µL onto a YED plate and repeated with a second YED plate.  We then exposed both plates to UV radiation for 5 seconds. We made a control plate that we did not expose to UV radiation and incubated all three plates for 3-5 days.  After that time, the lab professor removed them from the incubator.

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This is an interesting paragraph about the Yeast lab you were explaining. The only suggestion I have to make is to proof read and check grammer and punctuation.

Be sure to check your spacing when using numbers. A couple times, you didnt space between the numbers and the remaining sentence. For example "From the105dilution..." and "...~107to~104yeast cell..." both lac proper spacing.