DCPIP accepts electrons after they leave Photosystem II, but before they go to Photosystem I. Both photosystems are a part of the light-dependent reaction, which means that sunlight fuels their pathways. Photosystem II hydrolyzes water and pushes two electrons further into the electron transport chain. Instead of moving on to Photosystem I, DCPIP accepts these electrons. Without light, no electrons are pushed through the chain and therefore, DCPIP stays darker blue in color. If there are more chloroplasts exposed to light, there would be more electrons for DCPIP to accept, making it more clear in color. This relates to our prediction because if Kale does have more chloroplasts like we expect, then the absorbance of light should be lower.
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