pp

E-site: the ribosomal site harbouring deacylated tRNA on transit out from the ribosome. Describe reading frame and then mention when the ribosome knows what is the correct reading frame . Starts with AUG in P-site

-mention direction of ribosome reads mRNA (3’ to 5’ or vice versa)

-then introduce tRNA and function. point out anticodons and anticodon loops

-use tRNA, amino acid , mRNA, and ribosome manipulatives

-form the peptide bonds(discuss the location of amino and carboxyl groups involved) and mention the dehydration reaction

-ribosome reaches stop codon and the disassembly of the ribosome and release of polypeptide and mRNA

-highlight important of termination

-discuss genetic code and exceptions