Methods rough draft

Three main steps were involved in the mutagenesis of the yeast cells. A mutation was induced using UV radiation, initiating the experiment. Mutation was induced on wild-type yeast cells, a andα, which under normal conditions are able to synthesize the important molecules required for survival. Secondly, the yeast cells were selected for and screened for mutations. Screening was done by plating the original Yeast cells onto YED media. These plates were then exposed to UV light, damaging the cell’s DNA. Lastly, the mutants were categorized using complementation. 

In order to observe the effects of mutagenesis, dilutions of yeast cells were placed on a YED plates. The control plate contained a 10⁵dilution of cells to allow for comparison. A second plate, to be exposed to UV radiation contained a 10⁴dilution of cells. The plate containing a 10⁴dilution was then exposed in a UV irradiator for exactly twelve seconds. These plates were then incubated for a week at 30°C. Exactly one week later, to observe the effects of the induced mutagenesis, four mutant samples aW, aX, αy, and αZ and four known mutants, ADE1a, ADE2a, ADE1α, and ADE2α were obtained and streaked onto a YED plate. These were then crossed as shown in Figure 3. The plate was then placed in an incubator at 30°C for one day. After two days, the plate was replicated onto two MV media plates (one containing adenine and the other not containing adenine), and incubated for one day at 30°C.