TA – Manisha
Tuesday, 8 AM
Submitted: Oct, 30 2018
Isolation of Trimyristin from Nutmeg
Purpose:
The purpose of this experiment is to obtain a pure organic compound, trimyristin, from the natural source nutmeg. The techniques of extraction, distillation, recrystallization, and chromatography are used to isolate and purify the chemical compounds from the natural sources.
Reaction:
Trimyristin Myristic Acid:
MP: 56-57 ℃ MP: 54.4 ℃
MW: 723.16 g/mol MW: 228.37 g/mol
Experimental Procedure:
1.0 g of nutmeg was weighed out on a scale and transferred into a round-bottomed flask using a funnel. 3 mL of tert-butyl methyl ether and 3 boiling chips were also added to the RB flask. A distillations column and air condenser were set up with the black plastic connector to yield the highest results. The flask was lowered into the sand and monitored at a very gentle boil. The mixture was heated for 10 minutes, and then allowed to settle for a few minutes. The color of the mixture changed slightly to a lighter brown. Using a pipet, as much as of the liquid as possible was transferred to a test tube to settle further. The solids were left behind in the RB flask. The liquid was allowed to settle for a few minutes for even further separation of liquid ad small remaining solids. An empty 25 mL flask was pre-weighed. The liquid was transferred via pipet from the test tube to the clean 25 mL Erlenmeyer flask.
Filtration was performed by packing a glass pipet with a plug of cotton; the solution in the 25 mL Erlenmeyer flask was transferred via pipet and filtered through the cotton filter. The solution was allowed to drain completely before a new addition was performed. A pipet bulb was used to help speed up filtration. All of the solution from the Erlenmeyer flask was transferred.
2 mL of fresh tert-butyl methyl ethyl was added to the solid in the RB flaks and warmed very briefly to allow the solids to settle and the liquid was then transferred via pipet into the test tube and allowed to settle further. The liquid was then transferred from the test tube through the cotton pipet filter using a piper and into the same 25 mL Erlenmeyer flask as before.
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