Draft

Wild type C. violaceum will produces a purple colored antibiotic called violacein. Violacein production is regulated by quorum sensing. C. violaceum’s AHL synthase gene, CviI, synthesizes the signal molecule C6-HSL which then binds to the regulator protein CviR. The CviR/C6-HSL complex regulates an operon responsible for violacein production. On the Petri plate streaked with wild type C. violaceum, it was expected that the heavy band of inoculum at the top quarter of the plate would turn completely purple. The wild type strain was expected to produce violacein because the AHL synthase and regulator protein were not mutated. Thus, this strain could produce its own signal molecule and receptor to activate the operon responsible for violacein production.