Draft

The aim of this experiment was to explore the wide variety of organisms found in biofilms that form on toothbrushes. My lab partner’s used toothbrush was the source of these bacteria, and was sacrificed for the purposes of this experiment. The toothbrush head was cut off and placed into sterile saline to create a fluid suspension. An inoculum from the toothbrush-saline suspension was streaked onto TSA, SBA, MacConkey agar (MAC), Mitis-Salivarius agar, and CDC anaerobe blood agar plates. After incubating the plates, it was expected that each one would contain several different colonies that would allow the bacteria growing on the toothbrush to be directly observed.