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Draft

Submitted by cgualtieri on Thu, 11/08/2018 - 17:21

The results of this experiment were in accordance with my expected results. On the slide culture of P. aeruginosa, a thick, slimy, green goo had grown and covered the entire slide and surrounding glass dish. When I attempted to remove the glass slide from the dish, long thick strands of biofilm formed between the slide and the glass dish. It was like when you take a bite of cheese pizza and the cheese forms a long, gooey strand between your mouth and the slice. When I put the P. aeruginosa slide under the microscope at 400x, I could see long, thin, greenish grey strands of biofilm going in all different directions. It was clear from this slide that P. aeruginosa formed a biofilm that contained lots of EPS. At 1000x the strands were not as defined, but rod shaped bacteria with thin EPS and biofilm filaments in the extracellular space were clearly visible. This showed that P. aeruginosa is an excellent biofilm former, and can form biofilms in an artificial environment in the lab.

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