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In order to control the spider’s decision of choosing a side based on substrate color is it important to keep all other factors consistent. If in the experiment the spider forms a web it will be taken out in order to avoid the spider from choosing the same side due to having its web. Both sides of the environment should also be exactly the same except for background color. The temperature needs to be kept consistent inside of the tank. To do this, the spiders need to be kept away from windows and housed somewhere there is no incoming cold air. It has been shown that spiders are less active in colder temperatures, so keeping them at room temperature (70°F) is important. Light entering from outside of the tank should also be the same on both sides. To keep this consistent do not place in sunlight, or somewhere that there are shadows. Overhead lights should be used because they will keep the light even on both sides. The lights will follow an artificial light cycle in a lab setting with day and night.