Additionally, the results show that all of the insects become active beyond 20°C. Against the null hypothesis that the insects will show a mean activity count of 10, a T-test generates a p-value of 0.096, which is above 0.05 and therefore not statistically significant. Thus, these results imply that O. fasciatus is at its most active at and above 20°C, which is 5°C above the average daytime temperature in Massachusetts.
There are a number of plausible explanations for these results. Firstly, O. fasciatus covers a wide geographical range, from southern Canada to Costa Rica. Populations of O. fasciatus therefore inhabit different environments and different temperature ranges, and it may simply be that the milkweed bugs we used came from a population that inhabits a warmer climate. Therefore, the insects wouldn’t show the most activity in the Massachusetts average daytime range of 10-20°C but rather at higher temperatures above 20°C.
Alternatively, it may be that O. fasciatus needs a continuous source of food to maintain its body temperature at levels that promote activity and that only under higher heat conditions does its body temperature rise enough for it to become active. Given that the insects were starved 12 hours prior, it may be that they did not have the energy reserves or the necessary metabolites to heat themselves. Therein, when the ambient temperature was raised to 20°C above average daytime temperature, their bodies warmed up to a temperature that promoted activity, one they couldn’t reach due to a lack of energy. The primary confounding factor of this explanation being that they were provided with food during the temperature switching portion of the experiment, and yet none of the insects were seen to forage. However, this effect may have been due to the insects being put into new temperature and visual conditions during the temperature switch, thus motivating them to escape from the arena rather than search for food.
In summary, O. fasciatus activity is positively linearly correlated with temperature and is highest above 20°C. The data do not corroborate the hypothesis that the insects are most active at an intermediate temperature window of 10-20°C. Based on these results it may be the case that the level of O. fasciatus activity depends on its access to food, as it may need energy reserves to raise body temperature above daytime temperatures in Massachusetts in order to remain active during the day. Further research into O. fasciatus thermoregulation and potential life characteristic divergence among geographically distant O. fasciatus populations is necessary in order to elucidate the reasons behind these results.
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