The goal of this experiment is to determine the characteristics of the unknown mutants.
In this experiment, Yeast cells with unknown mutation that was made using UV light was crossed with other unknown mutant cells and other known mutants in order to characterize the unknown mutant cells. In this experiment, I crossed the unknown mutant colonies with known mutant colonies on an YED plate and then replicate the colonies on a MV plate and a MV+adenine plate. Using the unique characteristics of the different plates and the understanding of complementation, I analyzed the data to characterize the unknown mutants.
In the experiment that I conducted, in order to know the genotypes of the unknown haploid yeast cells, the unknown yeast cells were crossed with known mutant yeast cells used in previous experiments and with each other on an YED plate. Then the resulting plate was copied onto a MV plate and an MV+adenine plate. In general, the control was expected and there some crosses such as unknown alpha1 x ade 2a that produced a visible mutant, but the majority of the crosses resulted in complementation and as a result did not have a visible phenotype that could be seen in the YED plate. However, there were some mutation that was seen when a seemingly normal colony was transferred into an MV or MV+adenine plate.