Alzheimer's experiment

They used ELISA kits (used to detect and quantify proteins) to measure the beta-amyloid and tau, along with the TH. After processing, they injected the sample into the HPLC for analysis. T₄, T₃, rT₃ were detected in the HPLC. For the most part, there was no difference observed between AD and control groups. However, there was a lower correlation coefficient between CSF T₃ and blood T₃in AD patients as opposed to control. CSF TH and CSF beta-amyloid had no significant correlation. The absence of correlations showed the complex distribution of TH between blood and the CSF. . The same could be said for CSF T₄, T₃or rT₃between patients and controls. This was probably due to the fact that it was difficult to find controls who would want to opt for a lumbar puncture. They found significant negative correlations between rT₃, or rT₃/T₃ratio and MMSE only in AD patients.