Another specific aim the proposal has is to isolate and characterize the expression of highly conserved genes identified in Specific Aim 1 from the Animalia kingdom. The expansion of our subject of interest from chicken, zebrafish, Xenopus, and newt to the Animalia kingdom will allow for the comparison of the HOX gene in all vertebrate and invertebrate animals, which is different from species to species. Characterization methods of our data include a phylogenetic tree and a heat graph of gene expression. A modified phylogenetic tree allows us to map where organisms may have originated based on HOX similarities. The phylogenetic tree will take into account the Animalia kingdom.
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