Draft #37 Immunofluorescence organelle identity verification

In order to conclusively identify the organelle tagged with the mCherry fluorophore, we used immunofluorescence to visualize the hypothesized organelle. The mCherry tag was hypothesized to be on the nuclear lamina, so we stained the nuclear lamina with a GFP antibody. This allowed us to perform microscopy on fixed cells and see the colocalization of the mCherry tag and GFP stain. Because the antibody used was a known nuclear lamina antibody, its colocalization with the mCherry tagged molecules proves that the tag is on the same molecule as the antibody. Therefore the mCherry tag is in fact on the nuclear lamina.