Analysis of canine MC1R sequences is performed when MC1R sequencing from GeneWiz is received. First, the quality of MC1R gene sequences is checked to see if there is useable data. Sequencing files are modified using the sequencing program “4Peaks” to cut off unreliable ends of the files. Potential heterozygous sequences (two peaks on top of one another) are noted. BLAST results are retrieved to compare known sequences in the NCBI database with our dog sequence, noting Query cover, E-value, and percent identity of the top 5 hits. Our MC1R genome is then compared with Tasha the Boxer’s MC1R genome (the first dog to have its full genome sequenced) using EMBOSS Needle. Single nucleotide polymorphisms (SNPs) were noted and compared to specific MC1R SNPs identified in Nowacka-Woszuk, Salamon, Gorna, and Switonski’s paper (2012).
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