The hypothalamus releases thyroid releasing hormone (TRH). The TRH then goes to the pituitary gland which in turn releases thyroid-stimulating hormone (TSH). TSH then goes to the thyroid gland which produces T4, a biologically active molecule and T4 with is a biologically inactive pre hormone. both are released into the blood some times attached to a protein other times not attached to a protein, They thyroid hormone comes out of the blood vessel and through the blood-brain barrier through a transporter, such as MCT8. Then when inside of the cell, T4 is turned into T3. The T3 is then attached to the thyroid hormone receptor which attaches to the DNA along with coactivator or corepressor and causes the regulation of the gene. the thyroid hormone is important in changing the metabolism of the body and express developmental genes such as the notch signaling pathway. In the lab, the experiment that is trying to be done is how would changing the T3 concentration in zebrafish changes the number of neural stem cells. While this study have been done in mice before, the lab aims to do it on zebrafish, which is something that has never been studied before. In the lab the hypothesis is that the addition of T3 to the fish would cause an increase in thyroid hormone and cause an increase in notch signaling and as a result, there would be more cell differentiation and as a result, there would be less neural stem cells than there would be otherwise.
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