experiment with large number of samples

I think that one of the most interesting things that I've encountered while working in a lab is how a large number of samples affect how the scientists behave around the samples. I have been in labs where the main experiment was to do PCR, and I've worked in labs where PCR is just a chore that no one else really wants to do. I think one of the biggest differences between the people who are still learning and the people who do this for a career is that people who do it as a career really does not care about the things that are not the most important things. for example, when teaching in the lab, one of the ways that the students do it is to make each master mix for a specific gene and then add to the DNA and everything is carefully calculated. This is different if a person does this for a living. They are much less likely to be careful and count the concentration of the cells. they are far more likely to just add enough that will give them the results that are needed to do the experiment that can actually be published because of this, when I worked in labs, I was told that I should have some idea of how much 1ul looks like in the pipette and be able to add that much instead of going back and forth between the DNA and the PCR tubes.  Being careful isn't as important as getting things done at work especially if there are a lot of resources and there is high pressure to get results like at a company.  Being careful is very good when starting out, but it quickly becomes a burden when there are over 100 PCR to do and that isn't even the main focus of the experiment. I have also taught people in trypsonizing the cell cultures that the amount that is added is whatever that will trypsinize the cells without killing the cells. I will usually just pour the reagent instead of taking careful measurements of it. this was a big change from how I did it at the beginning where I spent most of the time splitting cells and each time there was a new experience. now because the focus isn't n splitting the cells, I just do whatever is the fastest and still get the result that I want. I think that something like this is not really something that can be really be taught at a class but can only be learned through working in a lab. although, the idea of having a class where the main objective is to find better ways to do simple and common lab technique is interesting.