Draft

Today, in my neurobiology and physiology lab, we dissected zebrafish brains that were treated with 0.5 mM PTU and 300 nM T4. Drug treatment with PTU induces hypothyroidism, while treatment with T4 induces hyperproliferation. We are measuring how the different levels of thyroid hormone affects neural stem cell proliferaation. After dissection of the brain, we will mount themn and look under a fluorescent microscope to count indivial cells in the posterior recess and later recess. These two regions make up the hypothalamus which is where neural cell proliferation occurs.