discussion

In the MV and YED plate, there were many white colonies. The reason for the white colonies in the MV plates is very likely due to complementation. In the YED plates, it is possible that some of the white colonies have a mutation in ade2. However, because the adenine that is required to grow a colony of ade2 mutants is lacking in the MV plates, it is unable to grow. If a colony is growing on the MV plate it can be considered that the  Because the complementation only occurs if the mutations were on the same gene, this indicates that if the resulting diploid colony was white they do not have the mutation at the same gene. This indicates that unknown alpha 2 and the two unknown a do not have mutation at either ade1 or ade 2 since the resulting diploid cells white when crossed with both ade 1 and ade 2.