In the cross between the known mutants and unknown 1a, the expectation was that since the unknown 1a had a red color that is similar to that of ade2a, in the cross between unknown 1a and ade2alpha the cross would result in a red colony of diploid colony, and the cross with ade1alpha would result in a white diploid colony. While the cross with ade1 alpha was white as expected, the cross with ade2alpha was also white which was unexpected (figure 1, table1). In the replica of the colonies in the MV plate, the expectation was that the cross between unknown 1a and ade2alpha would not be able to grow well on the MV plate while the cross between unknown 1a and ade1alpha would be able to grow well on the MV plate. While unknown 1a did not grow well on the MV plate, both of the diploid colonies resulting from unknown 1a grew well on the MV plate as shown in figure 2 and table 2. In the MV+adenine plate replica, the expectation was that both of the diploid colonies would be able to grow on the MV+adenine plate. The result was as expected with both of the diploid crosses from unknown 1a and known mutants growing well on the MV+adenine plate as shown in figure 3, table 3.
In the cross between the known mutants and unknown 2a, the expectation for the cross on the YED plate was that since the unknown a is a pink haploid colony, the cross with ade1 would create a visibly pink diploid colony whereas in the cross with ade2, there would be complementation and result in an white colony. However, neither of the crosses resulted in a visibly mutant colony as shown in figure 1 table 1. When the colonies were transferred to the MV plate, the expectation that the diploid cross with ade1a would not be able to grow well. However, both of the diploid crosses grew well on the MV plate, as shown in figure 2 and table2. In the MV+adenine plate, the expectation is that both of the diploid crosses would grow well. The result was as expected, as both of the colonies grew well on the MV+adenine plates, as seen in figure 3 and table 3.
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