The goal of this study is to determine whether the effects of T3 on progenitor cell proliferation and oligodendrocyte maturation are causally related or instead are independent. O-2A are biopotential glial progenitor cells. A2B5+ marker for presence of O-2A (biopotential glial progenitor) cells. GC+ is a marker that is expressed by mature oligodendrocytes.Table 1 shows the percentage of glial progenitor cells and mature oligodendrocytes that are present in the cell cultures that has the T3 added and the control. The control numbers mean that when there is no T3 present, there are around 75% glial progenitor cells and 6.8 mature dendrocytes 6 days after incubation. The T3 numbers means that 6 days after the T3 was added there were 24 percent glial progenitor cells and 57 mature oligodendrocytes, indicating that the presence of T3 causes the cells to mature into mature oligodendrocytes. Table 2 indicates that T3 blocks proliferation. The data is showing that T3 blocks the proliferation by marking the glial progenitor cells that have taken up BrdU. Because the percentage of BrdU in A2B5 labeled cell was twice as much in control as compared to the T3 treated cells. This indicates that the amount of A2B5 that are going under S phase, and as a result also indicates cell proliferation. Because the proliferation is twice as high in the control, the data is demonstrating that the proliferation is lower in the T3 cells. Because all other factors are kept constant, this table shows that T3 is what is causing the cells to stop proliferating.
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