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Hershey and Chase

Submitted by smomalley on Fri, 09/20/2019 - 11:44

The Hershey and Chase experiment is vital in the study of genetics. This ecperiment prooved that DNA is the hereditary material in bacteria and bacteriophages. This experiment radioactively labeled the phosphorous in DNA, and the sulfer in protein in a bacteriophage. The bacteriophage was allowed to infect a bacterial cultrue. Hershey and Chase used a blender to separate the bacteriophage from the bacteria, after the infection. They then placed the cells in a centrofuge and let the materials separate by weight. The heavier bacteria fell to the bottom of the centrofuge to form a pellet, while the lighter bacteriophage was suspended in the supernate. The radioactively labeled phosphorous in the DNA was found in the pellet, the radioactively labeled sulfer was found in the supernate. This proved that the DNA was the hereditary material of the bacteriophage, and baceria, and not the protein which remained outside of the bacteria.