Experiment steps for Neurobio

Before beginning a loss of function experiment, the exact location needs to be identified on the mouse brain atlas. Once this area is identified, the coordinates for this location will be calculated. The coordinates will be inputted into a sterotaxic device and a lesion will be made in the mouse. Once the lesion is made, the mouse will be observed for behavioral difference over a set period of time. At the end of the experiement, the mouses' brain will be collected and preserved with formadehyde. It with then be fixed, frozen and placed in a vibratome to be sliced into either a sagittal, coronal or horizontal samples. The sample will then be stained using IHC (immunohistochemistry). This will give scientists the ability to see exactly what happened in the brain during the experiment.