Today in my neurobiology and physiology lab, we drug treated 5 days post fertilization zebrafish with 0.5 mM PTU and 300 nM T4 to manipulate the levels of thyroid hormones in the zebrafish. Also, we drug treated 7 days post fertilization zebrafish with 1X EdU for three hours in a 28 degress Celsius incubator. During the three hour wait, we were lectured on the hypothalamus-pituitary thyroid axis and the Notch signaling pathway to help us better understand the whole process when formulating our hypothesis. Along with this information and additonal readings, we will have to formulate our own hypothesis and test for it in the upcoming weeks.
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