Draft 2

To determine the cytoplasmic polarity, anterior side had Mex-5 protein at a higher concentration. The protein would undergo redistribution across the cytoplasm. Par-1 (posterior) phosphorylates MEX-5 and it speeds up the diffusion rate and so it is rich in the anterior side. They proved that Par-1 is necessary and sufficient by doing knockdowns of Par-1 alleles. Mex-5 had a larger complex which were more sluggish in anterior side comparison to the posterior side. The smaller complex diffused much faster from posterior on to the anterior. In terms of a model, this was described as Par-1 phosphorylating the Mex-5 to turn it into the fast species to increase the diffusion rate.