According to the review paper, the timing of thyroid development is happening during 2nd day, and thyroid hormone production starts after the third day. This means that the experiment is done after the fish has been producing a normal amount of thyroid for about two days.
Neurogenesis: neurogenesis seems to occur from day 1 to day 3 after fertilization. The experiment is done after neurogenesis has been happening for a few days. In zebrafish, it is also notable that adult zebrafish have more active neurogenesis compared to mammalian species.
Oligodendrogenesis: oligodendrogenesis seems to occur from day 2 to day 7 after fertilization, meaning that the Thyroid hormone is added while it was occurring.
The experiment is different from other experiments that I have read about because in the other experiments, while it is established that PTU deiogenizes, causing thyroid hormone decrease, the effects of PTU on developing zebrafish brain has not been studied enough. In addition, the effect of hypo or hyperthyroidism is have never studied in zebrafish adult or developing brain. Most of the research on hyper and hypothyroidism in developing brains came from humans who suffer from some illness that causes overproduction or underproduction of thyroid or data that was obtained by artificially reducing or increasing thyroid hormone in mice. Our experiment would be different because it would specifically be looking at hyper or hypothyroidism in developing the zebrafish brain which there is very little data that has been gathered previously.
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