The size of the digested bands must be looked at and referred to the paper by both Berryere et al.’s paper (2005) and Schmutz et al.’s paper (2002), to analyze the gels performed for Agouti and TYRP1. Counting down from the 500 bp band, we cannot see the 100 bp band. A reason for this is that it may have traveled too far down the gel for us to see it. The Agouti gel does not show bands below 200. Berryere et al. (2005) says that "After digestion with BsmA1 the ay-allele yields fragments of 42, 90, 153 bp; other alleles yield fragments of 42 and 243 bp.” Since our digested band yields a length of around 200-300, we can conclude we do not have the ay allele, but still may possess the aw, a+, or a allele. The TYRP1 gel shows bands at 73 bp (visible) and 48 bp (not visible but assumed using paper) for MnIl, and bands of 93 bp (visible) and 28 bp (not visible but assumed using paper) for Acil. Schmutz et al. (2002) says that Acil digestion causes “animals with the premature stop codon cut to bands of 28 and 93 bp” while MnlI digestion causes “animals without the proline deletion cut to band sizes of 48 and 73 bp.” From this, we can conclude that our dog is mutant for the premature stop codon in exon five, and wildtype for the proline deletion in exon five. The study states that “all 43 of the brown group carried two or more of these sequence variants,” while “0 of 34 in the black group carried two or more of these variants” (Schmutz et al., 2002). If our dog has the third variant, we cannot confirm whether the dog will be black or brown from the TYRP1 analysis. However, the variant in exon two is rarely found, suggesting that our dog is more likely to be black in coat color. If we could select one more allele to analyze, we would analyze this third rare variant in exon two because it would conclude whether our dog is black or brown. Analysis of the third variant would be performed by seeing if there is “a serine substitution of a cysteine at amino acid 41 in exon 2” (Schmutz et al., 2002).
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