This is the updated methods for what we did (or at least what I did)
Approach and Analysis
This experiment aims to sample leaves from every available Elm species on the University of Massachusetts Amherst campus. There are currently 293 Elms on campus, and 16 unique species (Beals). When considering unique species, the Smooth Leaf Elms, a and b, were considered one species, and spp. was ignored because the species cannot be identified. Two of each Elm species will be selected through the use of a random number generator. The selected Elms will be searched for evidence of leaf miners by observing any accessible branches of the tree. Once a leaf miner is found, a circular marking sticker will be placed on the leaf, the next 9 closest leaves will be documented for leaf miner presence, and the branch will be marked with flagging tape. A picture will be taken with the trees identification number, and with all ten leaves visible in the same plane of view. A second picture of the entire tree, with the flagged branch visible, and the trees designation number, will also be taken. After, the leaf with the median amount of leaf mines will be collected in a single use marked bag for further analysis. All observed leaf miner larva will be collected from the infected tree, and placed in the bag with the infected leaf.