Schedule

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*Reading and Due Dates*
Reading Special Instructions Wiki Results Up Lab Report
Tuesday 01/24 Lab 0.1: Introduction to micro-pipetting: Micro-Mixology
Thursday 01/26 Lab 1.1: DNA Extraction
Basic Molecular Biology (pp 5-19, Dale and von Schantz, 2002), http://tinyurl.com/jx7cwgy
Purification and Separation of Nucleic Acids (pp 30-39, Dale and von Schantz, 2002), http://tinyurl.com/jx7cwgy
Tuesday 01/31 Lab 1.2: DNA Quantification
Thursday 02/02 Lab 0.2: Introduction to Wiki Lab 1 Wiki due at end of period
Tuesday 02/07 Lab 2.1 Initial Working Maps
Genes and Genomes; Genes within a Genome (pp 39-43, Reece, 2004)
Understanding a Genome Sequence (pp 1-9, Brown), http://www.ncbi.nlm.nih.gov/books/NBK21136/
Nucleases (pp 6-9, Brown, Genomes), http://www.ncbi.nlm.nih.gov/books/NBK21129/#A6021
Thursday 02/09 No Class/ snow day Lab 1 Report due (02/10, 11 pm)
Tuesday 02/14 Lab 2.2 Finalizing Gene Models
Thursday 02/16 Lab 2.3 Choosing PCR Primers
Polymerase Chain Reaction (pp 143-148, Dale and von Schantz, 2002), http://tinyurl.com/jx7cwgy
Polymerase Chain Reaction (pp 153-167, Reece, 2004)
Tuesday 02/21 Lab 2.4 Testing PCR Conditions
Estimating the Size of DNA Fragments (Dorfman)
Thursday 02/23 Lab 2.5 Restriction Mapping Lab 2 Wiki due at end of period
Think Like a Geneticist
Transgenic plants: Agrobacterium (pp. 511-514)
Protein Function and Malfunction in Cells (Griffiths, et al, 2002), http://www.ncbi.nlm.nih.gov/books/NBK21297/
Tuesday 02/28 Lab 3.1 Identifying T-DNA Insertion Sites; Lab 0.3 Arabidopsis Essentials
Thursday 03/02 Lab 3.2 SALK Line Genetics Lab 2 Report due (03/04, 11 pm)
Tuesday 03/07 Lab 3.3 Phenotype Testing of Salk Mutant Lines
Pairwise Sequence Alignment (pp. 31-35, 47-49, Xiong, 2006)
Sequence Comparisons (pp. 195-208, Dale and von Schantz, 2002), http://tinyurl.com/jx7cwgy
Thursday 03/09 Lab 4.1 Basic Local Alignment Tools (BLAST) (transfer cellophanes) Lab Plant Structures due
Tuesday 03/14, Thursday 03/16 NO CLASS/ Spring recess
Tuesday 03/21 Lab 3.4 Identifying Genotypes of SALK Line Plants: DNA extraction and PCR
Thursday 03/23 Lab 3.5 Identifying Genotypes of SALK Line Plants: Analysis Lab 3 WIKI due at end of period
Genes and Genomes; Genes within a Genome (pp 317-324, Reece, 2004)
Analysis of the Transcriptome: DNA microarray technology(pp 412-424, Primrose et al., 2006), http://tinyurl.com/m52dgws
Tuesday 03/28 Lab 4.2 Library Workshop; Work on wiki
Thursday 03/30 Lab 4.3 Representing Sequence Similarity Data; Work on wiki Lab 4 Wiki due at end of period
Tuesday 04/04 Lab 5.1 Microarray Resources Lab 3 Report due
Thursday 04/06 Lab 5.2 Statistical Analysis of Microarray Data Lab
Doing Statistics with Excel (Dorfman), Lab manual pp 24-38
Tuesday 04/11 5.3 RT-PCR Planning Lab 4 Report due
RT-PCR (pp. 177-182, Reece 2006)
Thursday 04/13 Lab 5.4 RNA Extraction and Quantification
Tuesday 04/18 NO CLASS/ Monday class schedule will be followed
Thursday 04/20 Lab 5.5 Reverse Transcription and PCR
Tuesday 04/25 Lab 5.6 Analysis of qPCR Reactions/Clean Up Lab 5 Wiki due at end of period
Thursday 04/27 Final Presentations (N610, Life Sciences Laboratory, 1-4 pm) Lab 5 report due (04/28, 11 pm)
Final Exam period