Schedule

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*Reading and Due Dates*
Reading Special Instructions Wiki Results Up Lab Report
Monday 01/23 Lab 0.1: Introduction to micro-pipetting: Micro-Mixology
Wednesday 01/26 Lab 1.1: DNA Extraction
Basic Molecular Biology (pp 5-19, Dale and von Schantz, 2002), http://tinyurl.com/jx7cwgy
Purification and Separation of Nucleic Acids (pp 30-39, Dale and von Schantz, 2002), http://tinyurl.com/jx7cwgy
Monday 01/30 Lab 1.2: DNA Quantification
Wednesday 02/01 Lab 0.2: Introduction to Wiki Lab 1 Wiki due at end of period
Monday 02/06 Lab 2.1 Initial Working Maps
Genes and Genomes; Genes within a Genome (pp 39-43, Reece, 2004)
Understanding a Genome Sequence (section 7.1, Brown), https://www.ncbi.nlm.nih.gov/books/NBK21136/
Nucleases (section 4.1.2, Brown), http://www.ncbi.nlm.nih.gov/books/NBK21129/#A6021
Wednesday 02/08 Lab 2.2 Finalizing Gene Models Lab 2 Wiki first part due at end of period Lab 1 Report due
Monday 02/13 Lab 2.3 Choosing PCR Primers
Polymerase Chain Reaction (pp 143-148, Dale and von Schantz, 2002), http://tinyurl.com/jx7cwgy
Polymerase Chain Reaction (pp 153-167, Reece, 2004)
Monday 02/20 NO CLASS/ Presidents' Day
Wednesday 02/22 Lab 2.4 Testing PCR Conditions
Estimating the Size of DNA Fragments (Dorfman)
Monday 02/27 Lab 2.5 Restriction Mapping
Think Like a Geneticist
Transgenic plants: Agrobacterium (pp. 511-514)
Protein Function and Malfunction in Cells (Griffiths, et al, 2002), http://www.ncbi.nlm.nih.gov/books/NBK21297/
Wednesday 03/01 Lab 3.1 Identifying T-DNA Insertion Sites; Lab 0.3 Arabidopsis Essentials
Monday 03/06 Lab 4.1 Basic Local Alignment Tools (BLAST) (Sterilize and plate seeds) Lab 2 Wiki due at end of period
Wednesday 03/08 Lab 3.2 SALK Line Genetics Lab 2 Report due
Pairwise Sequence Alignment (pp. 31-35, 47-49, Xiong, 2006)
Sequence Comparisons (pp. 195-208, Dale and von Schantz, 2002), http://tinyurl.com/jx7cwgy
Monday 03/13, Wednesday 03/15 NO CLASS/ Spring recess
Monday 03/20 Lab 3.3 Phenotype Testing of Salk Mutant Lines Lab Plant Structures due
Wednesday 03/22 Lab 4.2 Library Workshop; Work on wiki
Monday 03/27 Lab 3.4 Identifying Genotypes of SALK Line Plants: DNA extraction and PCR
Wednesday 03/29 Lab 4.3 Representing Sequence Similarity Data; Work on wiki Lab 4 Wiki due at end of period
Monday 04/03 Lab 3.5 Identifying Genotypes of SALK Line Plants: Analysis
Genes and Genomes; Genes within a Genome (pp 317-324, Reece, 2004)
Analysis of the Transcriptome: DNA microarray technology(pp 412-424, Primrose et al., 2006), http://tinyurl.com/m52dgws
Wednesday 04/05 Lab 5.1 Microarray Resources
Monday 04/10 Lab 5.2 Statistical Analysis of Microarray Data Lab Lab 3 WIKI due at end of period Lab 4 Report due
Doing Statistics with Excel (Dorfman), Lab manual pp 24-38
Wednesday 04/12 5.3 RT-PCR Planning
RT-PCR (pp. 177-182, Reece 2006)
Monday 04/17 NO CLASS/ Patriot's Day
Tuesday 04/18 (Monday class schedule) Lab 5.4 RNA Extraction and Quantification Lab 3 Report due
Wednesday 04/19 Lab 5.5 Reverse Transcription and PCR
Monday 04/24 Lab 5.6 Analysis of qPCR Reactions
Wednesday 04/26 Work on Wiki/Final Presentations/Clean Up Lab 5 Wiki due at end of period
Final Exam period Presentations and Lab 5 report due