Methods and Materials
Two plates containing PpNH4 media were supplied by Professor Bezanilla’s Laboratory. The media contains the following macro nurtients: MgSO4 1.03mM, KH2PO4 1.86 mM, Ca(NO3)2 3.3 mM, Di-ammonium tartrate 2.72 mM, and FeSO4 45 μM. The media also contains the following in trace amounts: H3BO3 9.93 μM, CuSO4 220 nM, MnCl2 1.966 μM, CoCl2 231 nM, ZnSO4 191 nM, KI 169 nM, and NaMoO4 103 nM. The growth solution was mixed with 7 g/L of agar to create a solid growth media. This growth mediate works best with the moss Physcomitrella patens, but was used in this experiment as a good enough growth medium for an unknown species of moss.
Temperature Recording Equipment:
An indoor/outdoor thermometer was placed next to the specimens in the appropriate location to accurately collect temperature data for both conditions. Temperatures were recorded in degrees Fahrenheit. The temperature was monitored and recorded throughout the day by various member of the BCRC staff. Monitoring of the temperatures started on the morning of Tuesday, April 18th and ended on Friday, April 21st at 2:00 pm.
The moss samples were collected from the Durfee Conservatory on the University of Massachusetts Amherst campus. Permission was granted by the staff member in charge and the collection was conducted by the staff member. The collected moss was placed on the two growth medium plates. Each large moss specimen was further divided into one large piece and five smaller sprouts of varying size of a smaller number of individual moss plants. These smaller pieces were embedded in the agar/growth medium. Each plate was photographed with a quarter as a size reference for evidence of initial size and condition.
One plate was placed in the window sill inside the BCRC and the other was placed just outside on the same window sill. The window was kept closed through the entirety of the experiment. The placement of the two plates was Friday, April, 14th at approximately 2:20 pm.
The experiment ended at 2:00 pm on Friday, April 21st. The moss plates were again photographed with a quarter as a reference for size. Each plate was analyzed for growth and overall condition.