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DNA replication: PP

Submitted by sjurgilewicz on Fri, 04/21/2017 - 11:17

When protiens interact with DNA, they interact on the major groove because the minor groove is too small. Nucleotides make up the DNA strand and form a polymer. Each nucleotide contains a phosphate group, a sugar, and a nitrogenous base. Base pairing occurs where A attaches to T and G attaches to C. The 1’ carbon is attached to base all the way to 5’ carbon at the phosphate in the sugar. This is important to the directionality of the polymer. The 3’ end of DNA/polymer is on the sugar, 5’ is where the phosphate is exposed. Polymers attach by hydrogen (non-covalent) bonding to create the DNA strand. The DNA runs antiparallel fashion. A primer is required for DNA polymerase to begin adding nucleotides.

RNA has a difference in its sugar as compared to DNA. The 2’ carbon has OH coming off of it, DNA has 2’ H. RNA is less stable, the oxygen allows it to act as a catalyst in chemical reactions. RNA can make intramolecular reactions on itself, like a protein, but structures are limited (ribosomes & splicasome). RNA has uracil instead of thymine. When DNA is damaged, cytosine can change to uracil and base paring then does not occur properly. It is possible for double stranded RNA to be formed and DNA can bind with it. It is a hybrid double helix, a slightly different shape. DNA replication is semi-conservative (a mix). Originally there were three methods thought up, Semi-conserve, Conservative and dispersive. 

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Comments

Great paragraph; it's very informative. I'd swap the order of some sentences around to make the information flow better. For instance, the first sentence talks about major groove and minor grooves. To the reader, the first sentence is the main idea. However, the paragraph goes on to explain information about DNA. Manybe move the first sentence to a later part of the paragraph or a completely new paragraph about DNA replication. Great job.

This is ok but the paragraph lacks a cohesive narrative its just kind of a random jumble of statemnts about DNA rather than a complete over veiw of DNA's properties. there are alos some factual errors in the paragraph, you say the 3' end is on the sugar and 5' end is where the posphate is, it kind of sounds like your saying there is no suagr at the 5' end when there is it's what the phosphate is atached to. You also failed to mention the hydroxyl group at the 3' end. On top of that there are a few gramatical mistakes like when you say "The DNA runs antiparrelle fashion" instead of "the DNA runs in an antiparrelle fashion" I would proof read my paragraph nxt time If I were you.