A mixture of cyclohexane and toluene were separated using both simple and fractional distillation. The fractional distillation showed a much better separation than simple distillation, both proven in the temperature profiles and in the GC/MS taken. The packing of copper in the columns influenced the separation the most and is the main distinction that makes fractional more successful than simple distillation. To have better separation in the future, a longer column could be used as well as compounds with a larger boiling point difference. Specifically for fractional distillation, a column that is packed with more copper can increase chances of complete separation.