Creatine is one of the fast twitch muscles’ most basic form of energy storage. In the muscles, the creatine molecules bond to a phosphate group, which later provides the muscles with an immediate source of ATP replenishment during the first few seconds of intense exercise. By having a readily avalible supply of phosphate, ADP can quickly pair with a creatine's phosphate group, thereby forming new ATP. People supplement with creatine because studies have shown that increasing the body’s available supply of creatine phosphate can promote a faster regeneration of ATP between sets of high-intensity exercise. By allowing more sets to be accomplished, the theory is that combining the supplement with exercise will lead allow the maintenance of a higher training intensity throughout workouts, and therefore an increase in muscle mass.
The objective of this lab experiment is to observe the effects of creatine supplementation on the muscle of a mussel. Becuase the inside of the mussel's shell is composed of smooth muslce, we are interested to see whether the supplementation will have the same effect that it does on skeletal muscle in the human body. By studying the amplitude of contraction and duration of contraction with and without creatine supplementation, we hope to conclude whether or not the creatine causes a noticeable difference in the contraction of the mussel's muscle.
We predict that supplementing the muscle with creatine will allow the muscle twitch to reach a higher maximum stimulus amplitude and achieve a greater contraction time.