In the paper Growth and metabolism of larval zebrafish; effects of swim training, it was discovered that once zebrafish hit a certain age it is more difficult for them to acclimate to chronic swimming because their rate of oxygen consumption does not adjust to conditions of hypoxia as readily. In this specific experiment, that age was 21 days old. The data found that fish who had reached this age had significantly higher routine oxygen consumption and mass-specific routine oxygen consumption values after 8 and 11 days of being trained to swim at various numbers of body lengths per second, when compared to fish only 96 hours or 9 days old.
We found this paper to be interesting because it compared metabolic rates of fish based on their age and stage of development. The findings applied to the experiment conducted in our lab because all fish we used were adults, and therefore greater than 21 days old. Therefore, because our fish would be less able to adjust to hypoxic conditions, it would be very possible to observe changes in their metabolic rate based on significant differences in their oxygen consumption over time. We decided to apply this knowledge towards an experiment in which we would examine the effects of gender socialization on adult zebrafish.